A rural agricultural-sustainable energy community model and its application to Felton Valley, Australia

Energy and food security require a delicate balance which should not threaten or undermine community prosperity. Where it is proposed to derive energy from conventional fossil fuel resources (such as coal, shale oil, natural gas, coal seam gas) located in established rural areas, and particularly where these areas are used for productive agricultural purposes, there are often both intense community concern as well as broader questions regarding the relative social, economic and environmental costs and benefits of different land uses and, increasingly, different energy sources. The advent of mainstream renewable energy technologies means that alternative energy options may provide a viable alternative, allowing energy demand to be met without compromising existing land uses. We demonstrate how such a Sustainable Energy Rural Model can be designed to achieve a balance between the competing social goals of energy supply, agricultural production, environmental integrity and social well-being, and apply it to the Felton Valley, a highly productive and resilient farming community in eastern Australia. Research into available wind and solar resources found that Felton Valley has a number of attributes that indicate its suitability for the development of an integrated renewable energy precinct which would complement, rather than displace, existing agricultural enterprises. Modelling results suggest a potential combined annual renewable energy output from integrated wind and solar resources of 1,287 GWh/yr from peak installed capacity of 713 MW, sufficient to supply the electrical energy needs of about 160,000 homes, in combination with total biomass food production of 31,000 tonnes per annum or 146 GWh/yr of human food energy. The portfolio of renewable energy options will not only provide energy source diversity but also ensures long-term food security and regional stability. The Felton Valley model provides an example of community-led energy transformation and has potential as a pilot project for the development of smart distributed grids that would negate the need for further expansion of coal mining and coal fired power stations

Pharmacological activation of endogenous protective pathways against oxidative stress under conditions of sepsis

Funding The study was funded entirely by institutional funds.Peer reviewedPostprin

Citizen Science for Citizen Access to Law

This papers sits at the intersection of citizen access to law, legal informatics and plain language. The paper reports the results of a joint project of the Cornell University Legal Information Institute and the Australian National University which collected thousands of crowdsourced assessments of the readability of law through the Cornell LII site. The aim of the project is to enhance accuracy in the prediction of the readability of legal sentences. The study requested readers on legislative pages of the LII site to rate passages from the United States Code and the Code of Federal Regulations and other texts for readability and other characteristics. The research provides insight into who uses legal rules and how they do so. The study enables conclusions to be drawn as to the current readability of law and spread of readability among legal rules. The research is intended to enable the creation of a dataset of legal rules labelled by human judges as to readability. Such a dataset, in combination with machine learning, will assist in identifying factors in legal language which impede readability and access for citizens. As far as we are aware, this research is the largest ever study of readability and usability of legal language and the first research which has applied crowdsourcing to such an investigation. The research is an example of the possibilities open for enhancing access to law through engagement of end users in the online legal publishing environment for enhancement of legal accessibility and through collaboration between legal publishers and researchers

Comparison of walking performance over the first 2 minutes and the full 6 minutes of the Six-Minute Walk Test

BackgroundAlthough the Six-Minute Walk Test (6MWT), as recommended by the American Thoracic Society, is widely used as a measure of functional endurance, it may not be applicable in some settings and populations. We sought to examine, therefore, performance over the first 2 minutes and the full 6 minutes of the 6MWT. Specifically, we investigated completion rates, distances walked, test-retest reliability, and the relationship between distances walked over the first 2 and the full 6 minutes of the 6MWT.MethodsCommunity-dwelling children and adults age 3-85 years (n = 337) were asked to walk back and forth on a 15.24 meter (50 ft) course as far as possible without running over a 6 minute period. Test completion and the distance covered by the participants at 2 and 6 minutes were documented. The reliability of distances covered at 2 and 6 minutes was determined by retesting a subsample of 54 participants 6 to 10 days later. The relationship between distances covered at 2 and 6 minutes was determined for the 330 participants completing the 6MWT.ResultsAll 337 participants completed at least 2 minutes of walking, but 7 children less than 5 years of age ceased walking before 6 minutes had elapsed. For the remaining 330 participants the mean distance walked was 186 meters at 2 minutes and 543 meters at 6 minutes. The distances covered at 2 and 6 minutes were reliable between sessions (intraclass correlation coefficients = 0.888 and 0.917, respectively). The distances covered over 2 and 6 minutes were highly correlated (r = 0.968).ConclusionsThe completion rate, values obtained, test-retest reliability, and relationship of the distances walked in 2 and 6 minutes support documentation of 2 minute distance during the 6MWT. The findings also provide support for use of a Two-Minute Walk Test as the endurance component in the Motor Battery of the NIH Toolbox

Mitochondrial function in heart failure: The impact of ischemic and non-ischemic etiology

Background Although cardiac mitochondrial dysfunction is associated with heart failure (HF), this is a complex syndrome with two predominant etiologies, ischemic HF (iHF) and non-ischemic HF (niHF), and the exact impact of mitochondrial dysfunction in these two distinct forms of HF is unknown. Methods and results To determine the impact of HF etiology on mitochondrial function, respiration was measured in permeabilized cardiac muscle fibers from patients with iHF (n = 17), niHF (n = 18), and healthy donor hearts (HdH). Oxidative phosphorylation capacity (OXPHOS), assessed as state 3 respiration, fell progressively from HdH to niHF, to iHF (Complex I + II: 54 ± 1; 34 ± 4; 27 ± 3 pmol·s− 1·mg− 1) as did citrate synthase activity (CSA: 206 ± 18; 129 ± 6; 82 ± 6 nmol·mg− 1·min− 1). Although still significantly lower than HdH, normalization of OXPHOS by CSA negated the difference in mass specific OXPHOS between iHF and niHF. Interestingly, Complex I state 2 respiration increased progressively from HdH, to niHF, to iHF, whether or not normalized for CSA (0.6 ± 0.2; 1.1 ± 0.3; 2.3 ± 0.3; pmol·mg− 1·CSA), such that the respiratory control ratio (RCR), fell in the same manner across groups. Finally, both the total free radical levels (60 ± 6; 46 ± 4 AU) and level of mitochondrial derived superoxide (1.0 ± 0.2; 0.7 ± 0.1 AU) were greater in iHF compared to niHF, respectively. Conclusions Thus, the HF-related attenuation in OXPHOS actually appears to be independent of etiology when the lower mitochondrial content of iHF is taken into account. However, these findings provide evidence of deleterious intrinsic mitochondrial changes in iHF, compared to niHF, including greater proton leak, attenuated OXPHOS efficiency, and augmented free radical levels

Sleep and Inflammation During Adolescents\u27 Transition to Young Adulthood

Purpose This study investigated the extent to which multiple sleep dimensions are associated with inflammation during adolescents\u27 transition to young adulthood, a developmental period when sleep difficulties and systemic inflammation levels are on the rise. Additionally, the moderating roles of socioeconomic status (SES) and ethnicity were explored. Methods A total of 350 Asian American, Latino, and European American youth participated at two-year intervals in wave 1 ( n = 316, M age = 16.40), wave 2 ( n = 248 including 34 new participants to refresh the sample, M age = 18.31), and wave 3 ( n = 180, M age = 20.29). Sleep duration (weekday and weekend) and variability in duration (nightly and weekday/weekend) were obtained from eight nights of wrist actigraphy. Subjective sleep quality was assessed using the Pittsburgh Sleep Quality Index. Levels of C-reactive protein (CRP), a biomarker of systemic inflammation, were assayed from dried blood spots obtained from finger pricks. Results Multilevel models demonstrated that greater weekday/weekend sleep variability and worse sleep quality were associated with higher CRP; shorter weekend duration was associated with higher CRP only at younger ages. Shorter weekday duration was associated with higher CRP only among high-SES youth, whereas greater nightly variability was associated with higher CRP only among European American youth. Conclusions Aspects of poor sleep may contribute to the rise of CRP during adolescents\u27 transition to young adulthood, especially in earlier years. In addition, some sleep-CRP associations may vary as a function of youth\u27s SES and ethnicity

Choose your target.

Journal ArticleThe technology of modifying endogenous genes has recently been extended from mice to Drosophila and sheep. Concurrently, genomic sequencing is uncovering thousands of previously uncharacterized genes. Armed with today's technologies, what are our best options for delineating the functions of these new genes

Gene targeting in adult rhesus macaque fibroblasts

<p>Abstract</p> <p>Background</p> <p>Gene targeting in nonhuman primates has the potential to produce critical animal models for translational studies related to human diseases. Successful gene targeting in fibroblasts followed by somatic cell nuclear transfer (SCNT) has been achieved in several species of large mammals but not yet in primates. Our goal was to establish the protocols necessary to achieve gene targeting in primary culture of adult rhesus macaque fibroblasts as a first step in creating nonhuman primate models of genetic disease using nuclear transfer technology.</p> <p>Results</p> <p>A primary culture of adult male fibroblasts was transfected with hTERT to overcome senescence and allow long term <it>in vitro </it>manipulations. Successful gene targeting of the HPRT locus in rhesus macaques was achieved by electroporating S-phase synchronized cells with a construct containing a SV40 enhancer.</p> <p>Conclusion</p> <p>The cell lines reported here could be used for the production of null mutant rhesus macaque models of human genetic disease using SCNT technology. In addition, given the close evolutionary relationship and biological similarity between rhesus macaques and humans, the protocols described here may prove useful in the genetic engineering of human somatic cells.</p